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Plenary Speaker

Professor Wolfgang Fritzsche

Biography        

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   Prof. Dr. Wolfgang Fritzsche’s work is focused on the utilization of plasmonic effects for bioanalytical applications. Based on a long experience in the development of nanoparticle-based approaches for molecular detection with special focus on DNA, methods are developed that allow for a sensitive, label-free and multiplexed detection. This work is complemented with interest into novel effects in the interaction of molecular components with plasmonic nanostructures, and their use for biosensing. Besides authoring more then 150 peer reviewed publications and several invited book chapters, editing 10 books and co-authoring 2 monographs (h-factor 31),  Dr. Fritzsche has been/is steering and coordinating many national and international projects, was 2016-2018 president of the German Society of Cytometry, and is organizing a series of bi-annual international meetings in Molecular Plasmonics as well as DNA Nanotechnology.

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Bioanalytics using plasmonic nanostructures

 

Wolfgang Fritzsche

 

Leibniz Institute of Photonic Technology (IPHT), A.-Einstein-Str. 9, Jena, Germany

fritzsche@ipht-jena.de

 

Abstract: Novel requirements for bioanalytical methods emerge due to trends such as personalized medicine or pathogen monitoring in environment and food. Here, innovative tools for diagnostics are needed, to be used outside of dedicated laboratories and with less qualified personnel, at minimal costs. Plasmonic nanostructures promise to provide sensing capabilities with the potential for ultrasensitive and robust assays in a high parallelization, and without the need for markers. Upon binding of molecules, the localized surface plasmon resonance (LSPR) of these structure is changed, and can be used as sensoric readout [1]. This is possible even on a single nanostructure level, using optical darkfield detection introduced more than 100 years ago [2], as demonstrated for DNA detection [3]. In contrast to SPR, LSPR senses only in a very thin layer (on the scale of the particle diameter), resulting in an efficient background suppression [4].

In order to multiplex this approach, an imaging spectrometer based on a Michelson interferometer has been developed, able to readout a whole array of sensors in one step [5]. On the sensor side, microarrays of gold nanoparticle spots were fabrictaed using spotting of pre-synthesized gold nanoparticles [6].  Such chemically synthesized particles allow for a cost-efficient generation of highly crystalline particles as nanosensors; by using microfluidic approaches, a high quality and reproducibility can be achieved [7]. The functionalization of the various particle spots is realized by spotting thiolized DNA onto each spot separately. Using this approach, a multiplex DNA-based detection of fungal pathogens involved in sepsis could be demonstrated [8].

 

 

[1]   A. Csaki, T. Schneider, J. Wirth, N. Jahr, A. Steinbrück, O. Stranik, F. Garwe, R. Müller and W. Fritzsche, Philosophical Transactions A 369, 3483-3496 (2011).

[2]  T. Mappes, N. Jahr, A. Csaki, N. Vogler, J. Popp, W. Fritzsche. Angew Chem Int Ed 51, 11208-11212 (2012)

[3]  T. Schneider, N. Jahr, A. Csaki, O. Stranik and W. Fritzsche, J Nanopart Res 15, 1531 (2013)

[4]  J. Jatschka, A. Dathe, A. Csaki, W. Fritzsche, O. Stranik. Sensing and BioSensing Research 7, 62-70 (2016)

[5]  D. Zopf, J. Jatschka, A. Dathe, N. Jahr, W. Fritzsche, O. Stranik. Biosensors and Bioelectronics 81, 287-293 (2016)

[6]  A. Pittner, S. Wendt, D. Zopf, A. Dathe, N. Grosse, A. Csaki, W. Fritzsche, O. Stranik. Analytical and Bioanalytical Chemistry (2019) 411, 1537-1547 (2019)

[7]  Thiele M, Soh J Z E, Knauer A, Malsch D, Stranik O, Müller R, Csáki A, Henkel T, Köhler J M and Fritzsche W Chemical Engineering Journal 288 432–40 (2016)

[8]  A. Zopf, A. Pittner, A. Dathe, N. Grosse, A. Csaki, K. Arstila, J. Toppari, W. Schott, D. Dontsov, G. Uhlrich, W. Fritzsche, O. Stranik. ACS Sensors 4, 335-343 (2019)

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