The objective of my master thesis was finding a way to functionalize a variety of gold nanoparticles with a bacteriophage generated from phage display technique. This phage is able to bind to the antibody anti-beta amyloid 42, the principle hallomark of Alzheimer’s disease. We tested different buffers and conditions to find out the best procedure that lead to a reliable result. The interaction that occurred between the phage and gold nanoparticles (nanostars and nanorods) was revealed using Raman Spectroscopy and more specifically the SERS signal. Although the complexity of phage, we succeeded to result the functionalization and to eliminate all noises caused by buffers in Raman spectrum. This work can be considered as a first step toward the diagnosis of Alzheimer’s disease using a sophisticated approach which is nanobiotechnology.